Aggregation‐Assisted Three‐Photon Fluorescence Resonance Energy Transfer Boosts Phosphorescence for Deep‐Tissue Time‐Resolved Intravital Brain Imaging
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B Wu, Y Li, L Liu, Y Yang, H Wu, M He…
Advanced …, 2025
Wiley Online Library
Three‐photon phosphorescence microscopic bioimaging holds promise for deep‐tissue time‐resolved brain imaging with high spatial resolution and contrast. However, developing probes with bright phosphorescence and strong second near‐infrared (NIR‐II) three‐photon absorption suitable for biological applications remains a formidable challenge. Herein, a kind of fluorescence resonance energy transfer (FRET)‐based nanoparticles (NPFA‐PorPt NPs) is proposed by co‐encapsulation of a three‐photon absorbing aggregation‐induced …
