Polysaccharide biosynthetic pathway profiling and homologous expression of the phosphomannomutase gene in Sanghuangporus sanghuang based on multi-omics analysis
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DOI:
文献链接: https://www.sciencedirect.com/science/article/pii/S014181302505130X
其他信息:
X Wang, Y Li, S Zheng, F He, Y Yang, W Yang, J Pan, Y Liang, Y Mei
International Journal of Biological Macromolecules, 2025•Elsevier
Abstract
Sanghuangprous sanghuang is a valuable medicinal macrofungus with abundant active metabolites. Improvement of macrofungal strains to increase the production of metabolites, especially polysaccharides, has become a research priority. In this study, we sequenced the whole genome and transcriptome of S. sanghuang SS-01 strain, identified the genes of key enzymes involved in the polysaccharide synthetic pathway, and constructed a homologous recombinant strain to increase S. sanghuang polysaccharide (SSP) yield for the first time. The assembled genome is 37.83 Mb with 22 contigs, encoding 7266 predicted genes. Forty putative genes related to sugar metabolism were identified, and the proposed SSP synthetic pathway was mapped. Transcriptional profiling was performed under different culture conditions to investigate the importance of key gene expression
